Staining solution – Intensifier, Mordants Objective Question

1.What is true for staining solutions?

  • a) They may be prepared as concentrated stock solutions and diluted as per requirement
  • b) Stock solutions may be prepared in alcohol and are diluted with water
  • c) They generally contain low concentration of stain around 1%
  • d) All of the above

2.What is mordant?

  • a) A substance that form a soluble compound with a stain and fixes bacterial cell on the slide
  • b) A substance that form an insoluble compound with a stain and serve to fix color to the bacterial cell
  • c) A substance that form an insoluble compound with a stain and decolorizes the bacterial cell
  • d) A substance that form an insoluble compound with a stain and fixes bacterial cell on the slide

3.When mordant is applied, while staining the specimen?

  • a) Before staining
  • b) Along with the staining
  • c) After staining
  • d) All of the above

4.Which of the following compound/s function as a mordant?

  • a) Tannic acid
  • b) Salts of aluminium, iron, tin, zinc, copper and chromium
  • c) Iodine
  • d) All of the above

5.Which of the following staining employs mordant?

  • a) Capsule staining
  • b) Gram’s staining
  • c) Flagella staining
  • d) Both b) and c)

6.Staining with basic dye is known as:

  • a) Simple stain
  • b) Positive stain
  • c) Direct stain
  • d) All of the above

7.The outer surface of the bacterial cell has an overall acidic character because:

  • a) Large amount of nitro group is located on the cell surface due to acidic amino acids
  • b) Large amount of carboxyl group is located on the cell surface due to nucleic acids
  • c) Large amount of carboxyl group is located on the cell surface due to acidic amino acids
  • d) Both a) and b)

8.When methylene blue is added on to the cell surface, the exchange of ions takes place resulting into:

  • a) Hydrogen bond formation between MB+ ion and cell surface
  • b) Covalent bond formation between MB+ ion and cell surface
  • c) Ionic bond formation between MB+ ion and cell surface
  • d) Ester bond formation between MB+ ion and cell surface

9.Simple stain helps to show:

  • a) Presence of organism
  • b) Nature of cellular content in exudate
  • c) Reveal size, shape and arrangement of the organism
  • d) All of the above

10.In indirect staining:

  • a) Bacterial cells are stained by acidic dye
  • b) Bacterial cells are stained by leuco dye
  • c) Bacterial cells are not stained but are made visible against dark background by acidic dye
  • d) Bacterial cells are not stained but are made visible against dark background by leuco dye

11.Gram negative cocci are:

  • a) Staphylococcus
  • b) Streptococcus
  • c) Acidoaminococcus
  • d) Diplococcus

12.Which of the following is not a factor affecting Gram reaction?

  • a) Age of the cell
  • b) Chemical constituents of the cell wall
  • c) Thickness of the cell wall
  • d) Shape of the cell

13. Chelton and Jones concluded that Gram reaction was possible due to:

  • a) Presence of lipid in the cell wall
  • b) A factor which gives/imparts stability to the staining components with membrane effect
  • c) Both a) and b)
  • d) None of the above

14.Acid fast staining was developed by:

  • a) Hansen Jones
  • b) Paul Ehrlich
  • c) Josheph Lister
  • d) Louis Pasteur

15.Acid fast technique is used in the diagnosis of:

  • a) Tuberculosis
  • b) Leprosy
  • c) Cholera
  • d) Both a) and b)

16.The high lipid content in the Mycobacterium species is known as:

  • a) Muramic acid
  • b) Mycolic acid
  • c) Mucoseamine
  • d) Mucopeptide

17.Woodhaurt found virulent Mycobacteria to be:

  • a) Strongly acid fast then nonvirulent forms
  • b) Weakly acid fast than nonvirulent forms
  • c) Nonacid fast
  • d) None of the above

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