Phase contrast microscope Objective Question

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1. In order to observe intracellular organelles in living eukaryotic cells, which microscopy is useful?

  • a) Phase contrast microscopy
  • b) Dark field microscopy
  • c) Bright field microscopy
  • d) All of the above

2. An opaque disc with thin transparent ring in phase contrast microscope is called:

  • a) Abbe’s condenser
  • b) Dark field stop
  • c) Annular stop
  • d) None of the above

3. The condenser of phase contrast microscope additionally contains:

  • a) Dark filed stop
  • b) Diaphragm
  • c) Annular stop
  • d) Barrier filter

4. Which of the following device is found present in phase contrast microscope?

  • a) Phase plate
  • b) Exciter filter
  • c) Annular stop
  • d) Both a & c

5. In phase contrast microscope, light rays strike to the specimen are retarded by about:

  • a) 1/2 wavelength
  • b) 1.2 wavelength
  • c) 1/4 wavelength
  • d) 1.4 wavelength

6. Where the phase plate is placed inside the phase contrast microscope?

  • a) In the objective
  • b) In the condenser
  • c) In the eye piece
  • d) Below the condenser

7. In the phase contrast microscope, undeviated light passing through phase ring:

  • a) Is retarded by 1/4th wavelength
  • b) Is advanced by 1/4th wavelength
  • c) Is retarded by 1/2nd wavelength
  • d) Both a) & b)

8. Which kind of observation is found in phase contrast microscope?

  • a) Object appears darker against bright background
  • b) Object appears brighter against bright background
  • c) Object appears darker against dark background
  • d) Object appears brighter against dark background

9. For study of which of the following purpose, we can use phase contrast microscope?

  • a) Microbial movement
  • b) Phagocytosis
  • c) Structure like endospore &inclusion bodies
  • d) All of the above

10. Most of the optical theories regarding microscope were developed by:

  • a) Edward Jenner
  • b) Leeuwenhoek
  • c) Ernst Abbe
  • d) Zacharias Janssen

11. What is the other name of phase contrast microscopy?

  • a) Bright-phase contrast microscopy
  • b) Dark-phase contrast microscopy
  • c) Bright-dark-phase contrast microscopy
  • d) Light-phase contrast microscopy

12. Phase contrast was discovered by whom?

  • a) Fritz Zernike
  • b) Knoll &Ruska
  • c) V. K. Zworykin
  • d) J. J. Thompson

13. Which of the following is not an example of condenser?

  • a) Abbe’s condenser
  • b) Aplanic condenser
  • c) Abortive condenser
  • d) Apochromatic condenser

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