Immersion objectives Question

Important Question

1. What is the possible angle of largest cone of light (come from object) which can be obtained
using high power objective?

  • a) 62°
  • b) 134°
  • c) 82°
  • d) 150°

2. What is the refractive index of water, air and cedarwood oil respectively?

  • a) 1.0, 1.33, 1.51
  • b) 1.51, 1.33, 1.0
  • c) 1.33, 1.0, 1.51
  • d) 1.33, 1.51, 1.0

3. Which of the following is to be used to obtain a cone of light of about 134°?

  • a) Air
  • b) Water
  • c) Cedarwood oil
  • d) Any of the above

4. Two lines close together _ can be separated with an oil immersion objective and
numerical aperture of 1.4.

  • a) 0.50 µ
  • b) 0.25 µ
  • c) 2500 nm
  • d) None of the above

5. The the NA of the objective, the __ will be its resolving power.

  • a) Greater, greater
  • b) Smaller, smaller
  • c) Greater, smaller
  • d) Both a) and b)

6. Which of the following material contain refractive index approximately around 1.52?

  • a) Sandalwood oil
  • b) Crown oil
  • c) Cedarwood oil
  • d) All of the above

7. What do you mean by equivalent focus?

  • a) Objective lens system produces a real image of the object of the same size as it is produced by a simple biconvex lens
  • b) Objective lens system produces a real image of the object of the same size as it is produced by a simple biconvex or converging lens
  • c) Objective lens system produces a real image of the object of the same size as it is produced by a converging lens
  • d) Objective lens system produces a virtual image of the object of the same size as it is produced by a simple biconvex or converging lens

8. The working distance is always than the equivalent focus of _:

  • a) Less, objective
  • b) Greater, objective
  • c) Less, ocular
  • d) Greater, ocular

9. The working distance of objects may be easily determined by:

  • a) Knowing the wavelength of light source and multiplying it with numerical aperture
  • b) Noting the number of complete turns of the fine adjustment required to bring object in sharp focus
  • c) Noting the number of complete turns of the coarse adjustment required to bring object in sharp focus.
  • d) All of the above

10. Each turn of micrometer screw (fine adjustment) raise the objective about _:

  • a) 1 mm
  • b) 0.1 mm
  • c) 10 mm
  • d) 6 mm

11. If the object is covered with a cover sleep, the working distance will __:

  • a) Increase
  • b) Decrease
  • c) Will not change
  • d) None of the above

12. What is correct for cover slip placed on object present on the glass slide?

  • a) If cover slip is thicker than working distance of the objective, it will not become possible to get object in focus
  • b) If the cover slip is thin, it will be possible to get the object in focus
  • c) Focus of the microscope of the object covered with cover glass will be different from that of the uncovered object
  • d) All of the above

13. The the refraction of the covering body (cover slip), the __ will be the apparent elevation:

  • a) Greater, more
  • b) Greater, less
  • c) Lesser, more
  • d) None of the above

14. How will you calculate the apparent depth of the object below the surface of cover glass?

  • a) Index of refraction of cover glass
  • b) Reciprocal of index of cover glass
  • c) Index of refraction of cover glass multiplied by light wavelength
  • d) Total turns of micrometer screw to bring object in sharp focus

Answer

QusAnsQusAns
1c8a
2c9b
3c10b
4b11b
5d12d
6d13a
7b14b

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