Fluorescence microscope Objective Question

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1. What is the function of exciter filter?

  • a) To excite light rays passes through it
  • b) To remove light rays with larger wavelength
  • c) To convert short wavelength rays into large wavelength rays
  • d) To produce ultraviolet rays

2. The barrier filter will remove _ rays & only allow passing ___ rays:

  • a) UV, Visible
  • b) Visible, UV
  • c) UV, Infrared
  • d) Infrared, Visible

3. When any molecule absorbs radiant energy, it will become excited & release trapped energy as light. Which statement is true for this light?

  • a) The light will have shorter wavelength than absorbed wavelength
  • b) The light will have longer wavelength than absorbed wavelength
  • c) The light will have equal wavelength to that of absorbed wavelength
  • d) None of the above

4. Which light is used for illumination in fluorescence microscope?

  • a) Violet light
  • b) Blue light
  • c) UV light
  • d) All of the above

5. What is the other common name of epifluorescence microscopy?

  • a) Incident light fluorescence microscopy
  • b) Reflected fluorescence microscopy
  • c) Both of the above
  • d) Accidental fluorescence microscopy

6. What is the common most type of fluorescence microscopy?

  • a) Incident light fluorescence microscopy
  • b) Refraction fluorescence microscopy
  • c) Both of the above
  • d) None of the above

7. Which source is used to create illumination in fluorescence microscope?

  • a) Tungsten filament
  • b) White light bulb
  • c) Mercury vapor arc lamp
  • d) Plane polarized light

8. Name the mirror, used to direct light to microscope in fluorescence microscopy:

  • a) Chromic mirror
  • b) Dichromic mirror
  • c) Chromatic mirror
  • d) Dichromatic mirror

9. The special type of dye molecules used in fluorescence microscope are called:

  • a) Auxochromes
  • b) Fluorochromes
  • c) Chromophores
  • d) Fluorescents

10. Which of the following is not an example of fluorochromes?

  • a) Diamino -1- phenyl indole
  • b) Acridine orange
  • c) Fluorescein isothiocynate (FITC)
  • d) Rhodamine (tetramethyl rhodamine isothiocynate – TRITC)

11. Which of the following is not an example of fluorochromes?

  • a) Acridine orange
  • b) Diamidino-2-phenyl indole (DAPI)
  • c) Fluorescein isothiocynate (FITC)
  • d) Tetraphenoxyethyl rhodamine isothiocynate

12. Which of the following task is possible with fluorescence microscope?

  • a) Photosynthetic microbes can be seen without applying fluorochromes
  • b) Differentiation of live bacteria from dead bacteria
  • c) Pathogens can be observed through antibodies/probes labeled with fluorochromes
  • d) All of the above

13. How can we label microbes to view in fluorescence microscope?

  • a) Fluorochromes labeled antibodies
  • b) Fluorochromes labeled probes
  • c) Fluorochromes labeled cell constituents
  • d) All of the above

14. Which dye is selectively used to stain Mycobacterium species in a modified acid fast technique?

  • a) Diamidino-2-phenyl indole (DAPI)
  • b) Fluorescein isothiocynate
  • c) Auramine
  • d) None of the above

15. Which of the following fluorochrome selectively stains DNA?

  • a) Diamidino-2-phenyl indole (DAPI)
  • b) Rhodamine
  • c) Acridine orange
  • d) Both a & c

16. What is a measure of refractive index?

  • a) Light passes from one medium to another
  • b) A substance slows the velocity of light, direction &magnitude
  • c) Proportion at which the ray will disappear
  • d) The focus of light to form images

17. Epifluorescence microscope is used to visualize:

  • a) Live microbial cells
  • b) dead microbial cells
  • c) Motile microbial cells
  • d) Photosynthetic microbial cells

18. Barrier filter & exciter filter are parts of:

  • a) Confocal scanning laser microscope
  • b) Phase contrast microscope
  • c) Fluorescence microscope
  • d) Differential interference contrast microscope

19. Who is known as father of microscopy?

  • a) Leeuwenhoek
  • b) Robert Koch
  • c) Louis Pasteur
  • d) Robert Hooke

20. Why dichromatic mirror is used in fluorescence microscopy?

  • a) It reflects shorter wavelength light to specimen & allow light of longer wavelength to pass through
  • b) It produces 2 chromatic images of the object
  • c) There are two mirrors to produce chromatic light
  • d) It reflects longer wavelength light to microscope & allow light of shorter wavelength to pass through

21. Why some molecules illuminate on absorption of radiant energy?

  • a) They become stable &release light
  • b) They become excited &release trapped energy as light
  • c) They become excited &release stable energy as light
  • d) None of the above

22. Who won 2008 Nobel Prize in chemistry?

  • a) Osamu Shimomura
  • b) Martin Chalfie
  • c) Roger Tsien
  • d) All of the above

23. In which filed 2008 Nobel Prize was awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien?

  • a) Microbiology
  • b) Physiology and medicine
  • c) Chemistry
  • d) Physics

24. The GFP gene was isolated from jellyfish belongs to genus :

  • a) Aequorea
  • b) Agaricus
  • c) Gracilaria
  • d) None of the above

25. What is the full name of GFP?

  • a) Gray Fluorescent Protein
  • b) Green Fluorescent Protein
  • c) Gray Fluorescein Protein
  • d) Green Fluorescein Protein

26. GFP gene is fused with the gene of interest and can be detected by _ microscopy:

  • a) Confocal microscopy
  • b) Phase contrast microscopy
  • c) Florescence microscopy
  • d) All of the above

27. Identify the correct match:

Column A: Dye/sColumn B: Use
1. Acridine orange and  Diamidino-2-phenyl indole (DAPI)i) Often attached to DNA probes or to antibodies that bind specific cellular components
2. Fluorescein isothiocyanate (FITC)ii) Often attached to antibodies that bind specific cellular components
3. Tetramethyl rhodamine isothiocyanate (TRITC or rhodamine)iii) Stains DNA
  • a) 1 and ii), 2 and iii), 3 and i)
  • b) 1 and iii), 2 and i), 3 and ii)
  • c) 1 and i), 2 and iii), 3 and ii)
  • d) 1 and ii), 2 and i), 3 and iii)

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