Dark field microscope Objective Question

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1. In dark filed microscopy a hollow cone of light is focused on the specimen in such a way that:

  • a) Unreflected & unrefracted rays do not enter objective
  • b) Reflected & refracted rays do not enter objective
  • c) Unreflected & refracted rays do not enter objective
  • d) Reflected &unrefracted rays do not enter objective

2. Which of the following dye is used in Dark field microscopy?

  • a) Acridine orange
  • b) Saffranine
  • c) Crystal violet
  • d) None of the above

3. Which of the following is true for dark filed microscopy?

  • a) Unreflected & unrefracted rays do not enter the objective
  • b) Light reflected or refracted by the specimen forms an image
  • c) Living, unstained organisms are observed
  • d) All of the above

4. Dark filed microscopy is normally used for diagnosis of _____.

  • a) Typhoid
  • b) Malaria
  • c) Syphilis
  • d) Skin infection

5. Which of the following is not the part of dark filed microscope?

  • a) Abbe’s condenser
  • b) Barrier filter
  • c) Dark field stop
  • d) Objective

6. Which microscopic technique is useful to observe living unstained microbes?

  • a) Phase contrast microscopy
  • b) Dark field microscopy
  • c) Differential interference contrast microscopy
  • d) All of the above

7. Which microscopy is used to observe living microbes by changing the bway of illumination?

  • a) Dark field microscopy
  • b) Bright microscopy
  • c) Contrast field microscopy
  • d) None of the above

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