Confocal microscopy Objective Question

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1. The full form of CSLM is:

  • a) Confocal Scanning Laser Microscope
  • b) Contrast Scanning Laser Microscope
  • c) Contrast Scanning Light Microscope
  • d) Confocal Scanning Light Microscope

2. In CSLM, the light that forms an image comes from:

  • a) The plane of focus
  • b) The specimen above the focal plane
  • c) The specimen below the focal plane
  • d) All of the above

3. In CSLM the specimen can be:

  • a) Illuminated with LED and can be stained by fluorescent dye
  • b) Illuminated with laser beam and can be stained by fluorescent dye
  • c) Illuminated with LED and can be stained by mutagenic dye
  • d) Illuminated with laser beam and can be stained by mutagenic dye

4. In CSLM the aperture eliminates:

  • a) Stray light from parts of the specimen that lie above and below the plane of focus
  • b) Stray light from parts of the specimen that lie above the plane of focus
  • c) Stray light from parts of the specimen that lie below the plane of focus
  • d) Straight light from parts of the specimen that lie above and below the plane of focus

5. A computer interfaced with Confocal microscope shows different images:

  • a) Digitized images from each plane in the specimen that is examined
  • b) Digitized images from only one plane in the specimen that is examined
  • c) Differential images from each plane in the specimen that is examined
  • d) Digitized 3-D images from each plane in the specimen that is examined

6. Biofilms in medical field can be studied using:

  • a) Epifluorescence microscopy
  • b) Confocal scanning laser microscopy
  • c) Phase contrast microscopy
  • d) Differential interference contrast microscopy

7. What should be the approximate focal length (f) for CSLM?

  • a) 16 mm
  • b) 20 mm
  • c) 50 mm
  • d) 70 mm

8. CSLM Microscope uses one of the following combinations:

  • a) Laser rays, Fluorescence staining and Computer
  • b) Laser rays, Beams of positrons and Computer
  • c) X-rays, Fluorescence staining and Probe
  • d) X-rays, Scan pictures and Computer

9. What should be the approximate focal length (f) for CSLM?

  • a) 16 mm
  • b) 20 mm
  • c) 50 mm
  • d) 70 mm

10. What should be the approximate focal length (f) for CSLM?

  • a) 16 mm
  • b) 20 mm
  • c) 50 mm
  • d) 70 mm

11. The Confocal Microscope improves images in different ways:

  • a) Illumination of one spot at a time reduces interference from light scattering by the rest of the specimen
  • b) The aperture above the objective lens blocks out stray light
  • c) Illumination of different spots at a time reduces interference from light by the rest of the specimen
  • d) Both a and b

12. Confocal Scanning Laser Microscope is used to examine:

  • a) Fluorescent stained specimen
  • b) Mutagen stained specimen
  • c) Cytological stained specimen
  • d) Biochemical stained specimen

13. CSLM uses which one of the following combinations:

  • a) Laser beam, fluorescence staining and computer
  • b) Laser beam, positron beam and computer
  • c) X ray, fluorescence staining and probe
  • d) X ray, scan pictures and computers

14. How does CSLM improve images?

  • a) Illumination of one spot at a time, reduces interference from light scattering by the rest of the specimen
  • b) The aperture above the objective lens blocks out stray light
  • c) Illumination of different spots at time reduces interference from light by the rest of the specimen
  • d) Both a) and b)

15. CSLM is used to examine:

  • a) Fluorescent stained specimen
  • b) Mutagen stained specimen
  • c) Cytological stained specimen
  • d) Biochemical stained specimen

16. The major component of CSLM is:

  • a) An aperture placed above the objective lens
  • b) Above and below the plane of focus
  • c) On the side of the plane of focus
  • d) On to the plane of focus

17. CSLM is used to study ____:

  • a) Biofilms on medical devices
  • b) Colonization in parts of the body
  • c) Biomass in muscle tissues
  • d) None of the above

18. Identify the microscopy technique from below image:

Dark field microscope
  • a) Phase-contrast microscope
  • b) Dark-field microscope
  • c) Simple microscope
  • d) Electron microscope

19. What do you mean by “F” and “f” shown in the below image:

focal length
  • a) F – focal length, f – focal point
  • b) F – focal point, f – focal length
  • c) F – final length, f – final point
  • d) F – final point, f – final length

20. Out of figure A or figure B shown below, whose resolution will be higher?

resolution
  • a) A
  • b) B
  • c) Similar in both
  • d) Cannot decide from above image

21. Identify microscopy from below shown image:

phase contrast microscope
  • a) Phase-contrast microscope
  • b) Dark-field microscope
  • c) Simple microscope
  • d) Electron microscope

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